Hi, I have tried to make a scenario to explain this better. The main use of reset is to reset between samples or to "Stitch" together samples when in continuous mode.
In the Scenario Continuous Acquisition With Reset off the VI stores the last Array value in the 1st Sample (White) and uses it as the first Array Value in the 2nd Sample (Red). This effectively "Stitches" together the white and red waveforms in the graph. This helps with data processing as there is no erroneous data point in between samples. although not that important in the time domain it can really skew frequency type stuff.
In the Scenario Single shot Acquisition I have emulated a time delay between samples. With the reset off we can see that the VI attempts to "Stitch" together the white and red waveforms and we end up with a flase trigger. Hence when using single shot sampling or using the same vi to sample different waveform we would want to always reset this VI in between calls.
This is illustrated in the scenario "Trigger Single shot with reset On"