Thanks for your help, guys. And thanks for your hint Jim Krings. I will look into the link you gave and hopefully be able to solve the problem. Thanks once again. Will ask you all again if I'm stuck! :DHi guys,I have several questions that I hope someone can give me advice on.1.Jim Krings, you said that I don't really need to use NI Vision. Does it mean that I can just use Labview to solve this problem? I'm thinking to change the image to grayscale and then use Labview to count the darkest pixels for the area. How can I use Labview to do that?2.I have a video of the scope going into the throat. Can labview acquire three images while the video is running, calculate the area and the volume between the first and 3rd image?Many thanks for your time!