Hello, Following my investigations: I am less convinced that the problem comes from the program. I removed the USB cable connecting the module to the PC (It turns out that the cable had been installed contained 2 extensions that were hidden in a buy electric chute). The total length of 3m. This cable was nearby the 230V power cables of this cabinet. So I replaced this set of cables by the original cable module and I physically isolated from the 230V power cables. I also added a ferrite on the cable closest to the acquisition module and a second closer to the USB plug of the PC. I also turned off when the PC starts the Auto Power Device Monitor "NI". In case of disconnection and reconnection I had the message of this monitor indicates to me that the module was detected. So I assumed (this is a guess ...) if my app is running and if the USB connection is lost and then found and if this "NI" Device Monitor detects the reconnection of USB , maybe it could "block" the acquisition ???. It does not concern the loss of USB, but only phoénomène blockage of acquisition ... Last thing, on the PC that manages the acquisition, there was another application (foreign to LABVIEW) to retrieve information about a different type of equipment with a USB cable to emulate a serial port running with FTDI driver. I also fired this acquisition to eliminate potential conflict problem of address to another? I attached some pictures to my post, if someone has an idea, it is welcome. Thank you.