lkranker

I have decided to save as an 8-bit image instead of trying to achieve 16bit resolution. The contrast exports perfectly in tif, jpg, and png. I guess I am losing some resolution, but it is still satisfactory for my purposes. The Cast Image did eventually accomplish this purpose, though I was able to do it through a sub vi specific to my application. Thank you to everyone who helped me figure out what was going on and pointed me in the right direction!

Thank you for the quick responses. I apologize for crossposting. I am new and I must have missed the option when I posted and wasn’t aware that I did. I will be more careful in the future. The camera I am using is a Q-Imaging Retiga EXi which is a digital CCD camera which mounts on a microscope for live cell imaging. The streaming image to the front panel has great contrast/brightness/resolution, so I am pretty sure that the settings on the camera/microscope are correct for my purposes. The problem seems to occur only when I export. The image type is Grayscale I16 if that helps (before I export). When I do a histogram of the image the values are between 0 and 4095. I did not test this using MAX as I am not sure how to do that. I have now discovered that if I export to PNG instead of TIF, the contrast is perfect (exactly as in the streaming image). Does that help with understanding my problem? Unfortunately, PNG is one of the few image types that my post-processing software won’t open. I know I can convert it from PNG to TIF using other software (ex MATLAB), but I would rather just export to something besides PNG in the first place. oops...I forgot to attach the sample file inputs. For whatever reason it would not allow me to attach the tif format file. Therefore, I have attached two png files. test.png = an unaltered image exported directly from my vi test2.png = the tif file being exported (only converted into a png, but with low contrast maintained)

I have written a vi that currently acquires data from a camera. The image steams in continuously in 16bit. I have a case structure that snaps the image and exports a single frame of the streaming image to a tif format using "IMAQ Write TIFF file". The tif file that results however is extremely low contrast. I am guessing that I need a conversion before exporting to tif, but I am unsure how to implement that. I am considering using "IMAQ Cast Image". Is there a better way? I don't necessarily need to use tif, so if there is another better method, I would be open to suggestions. If I do need to use cast image does anyone have recommendations for "# shifts" or "image type" that would work well for tiff? Any and all suggestions would be greatly appreciated!!! (Cast Image is shown in the pic)