gammaray

There is an updated LibMPSSE library on FTDI's website. LibMPSSE-I2C Examples https://www.ftdichip.com/old2020/Support/SoftwareExamples/MPSSE/libMPSSE.zip If you replace the libMPSSE.dll in the project folder with the one from the following location it should work. It's possible that when you open the VI the run arrow will be broken. In this case you need to set the Library Name for the Call Library Function Node in each subVI. I've just tried it, and it works.

By the way, this library is a bit weird.I haven't been able get the master yet to send an ACK when reading, if more bytes need to be read and a NAK after the last byte has received.

Hi, It was long ago when you asked. You've probably already solved it. I just started using the FT2232H in LabVIEW and noticed your question. Connect the DO and DI pins to each other and it will work.