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Why would you want to involve LabVIEW in this? I was going to suggest some kind of remote desktop solution, where users can login with their credentials and access the PC one at a time. But even if you were able to have 3 users all logged in at once, and all working at once, the hardware will be locked by one application by one user, and the other users, and other applications won't be able to access it.

Personal choice. Most documents you'll see from NI use the while loop, but I like being able to read, at the start of the loop, "This will only execute once." The stop terminal for the while loop can drift around a bit, and even if it was fixed in place, it's still at the right side of the loop instead of the left side. Wiring the N with a 1 means I recognize this type of loop as soon as I start reading it, instead of scanning across it to see the constant wired to the stop terminal.Under the hood, my understanding is that they both convert to the same assembly code, so it doesn't matter which you use.